Data Availability StatementAll data generated or analyzed in this research are one of them content. could obviously inhibit the upregulation level of antiapoptotic protein CRYAB of HMG rats and promote mammary gland cell apoptosis of HMG rats via increases of promoting apoptosis protein caspases-3, 8, and 9 and Bax and tumor suppressor protein p53, decreases of antiapoptosis protein Bcl-2, and release of cytochrome c. These results suggested that RuXian-I has protective and therapeutic effects on HMG rats induced by estrogen and progestogen possibly via promoting apoptotic pathway regulated by CRYAB and is a promising agent for treating HMG. 1. Introduction Hyperplasia of mammary gland (HMG) is the most common breast disease in middle-aged women worldwide . Susceptibility of HMG is related to many factors, including menstrual cycle, breastfeeding, occupation, abuse of sex hormone brokers, diet, and mental pressure . In recent years, the number of patients with the noncancerous benign diseases is usually increasing, and the morbidity is usually enhancing quickly, with a much higher risk of causing mammary carcinoma . Unfortunately, they are easily neglected because much more attentions have been paid to malignant lesions of the breast, breast cancer, for instance . Therefore, attention to breast hyperplasia is usually imminent. And it is significant for human health to discover more convenient, cheaper, and effective new therapeutic remedies with few side effects for treating HMG and to explore the anti-HMG mechanisms of these remedies for blocking its development to breast cancer. Growing attention to treat both breast malignancy and HMG has been paid to chemical brokers, including estrogen therapy , and surgical excision . Surgical treatment of patients is usually difficult to end up being recognized generally, while chemical substance agents bring many unwanted effects and complications and high recurrence rate often. However, significant amounts of analysis has been executed on the original Mongolian medicinal formulation in Internal Mongolia of China, which includes unique treatment options in many illnesses . Mongolian doctors think that the breasts hyperplasia disease is one of the Mongolian medical Qi su bu ri le du sen and He yi Diltiazem HCl bu ri le du sen disease types. The trigger is because of menstruation generally, especially postpartum, insufficient diet, incorrect living, or disorderly usage of medications and poor lactation, etc., which trigger the imbalance from the three root base of He yi, Sheila fever, Yellow Drinking water, and Ba da gan in the physical body, impacting the standard decomposition of dregs and essence. This creates diseased bloodstream, Sheila fever, and yellowish water. Beneath the actions of He yi, the breasts was swollen to create the breasts Qi su bu ri le du sen disease. The primary clinical manifestations certainly are a lump, discomfort, nipple release, and irregular menstruation, upset and chest Diltiazem HCl tightness, insomnia, and more dreams. The disease is usually prone to recurrence and persistence, which seriously affects women’s physical and mental health. Therefore, Pingqi blood circulation and Huoxue Tongluo have curative effects on breast hemorrhage and mastoma of gynecology. The traditional Mongolian medicine RuXian-I is also known as Hu hun e ru le which consists of 30 classic Mongolian medicines. It has the functions of calming Qi and blood, promoting blood circulation, resolving essences, and dross decomposition. It can promote the blood flow of the body and has the effects GCN5 of regulating menstruation, activating blood, analgesic, swelling, Diltiazem HCl soothing the nerves and nourish. Therefore, it has a very good effect on breast hyperplasia in women [8, 9]. Although there is a good effect, little is known about the mechanism. The previous study has revealed RuXian-I in the treatment of HMG affected the expression of seventeen proteins recognized via using proteomics. Among these, RuXian-I significantly downregulated.