On the other hand, the fact that the B19V genomic sequence was found to be statistically more frequent in patients with AITDs and non-AITDs tissue DNA samples (= 0.0231 and 0.0001, respectively) than in the control group individuals whose histories did not show of any Cinnamyl alcohol autoimmune or thyroid diseases, allows us to think that B19V is not only just a bystander, but could be implicated in thyroid diseases. (IQR: 22.50C756.8) and 43.00 copies/g DNA (IQR: 11.50C826.5), respectively. The viral load in one of the 35 nPCR B19V-positive thyroid tissue samples from the deceased subjects was 13.82 copies/g DNA. The viral load Cinnamyl alcohol in the tissue of patients with AITDs was higher than in whole blood, which possibly indicates B19V persistency in thyrocytes (= 0.0076). The fact that the genoprevalence of B19V NS was significantly higher in patients with non-AITDs compared to the control group and in the thyroid gland tissue of patients with AITDs, and that the non-AITDs viral load was higher than in tissue derived from the control group individuals, suggest the possibility that B19V infection could be involved in the development of thyroid gland diseases. 0.05) was considered as a statistically significant difference. 3. Results 3.1. B19V Serology by ELISA Specific anti-B19 IgG antibodies were detected in 35 (70%) out of 50 patients with autoimmune thyroid gland diseases (AITDs) and very similar rates Cinnamyl alcohol were detected in the group of patients with non-autoimmune thyroid gland diseases (non-AITDs)51 (67.1%) out of 76 patients, without a statistically significant difference between the groups (= 0.8454). None of the 76 patients with non-AITDs was positive for B19V IgM, while among patients with AITDs, one had virus-specific IgM and IgG simultaneously. 3.2. B19V NS Detection by Nested Polymerase Chain Reaction All the DNA samples were positive for -globin PCR and were therefore eligible for further study. The B19V genomic sequence was found in blood and/or thyroid tissue DNA samples in 14 out of 50 patients with AITDs (Figure 1)in 9 (64.3%) patients in thyroid gland tissue DNA samples only, in 4 (28.6%) patients in blood DNA samples only, in 1 (7.1%) patient in both the blood and tissue DNA samples. The B19V genomic sequence was detected in 35 out of 76 blood and/or thyroid tissue DNA samples from patients with non-AITDs (Figure 1)in 25 (71.4%) patients in the thyroid gland tissue DNA samples only, in 5 (14.3%) patients in the blood DNA samples only, and in Cinnamyl alcohol 5 (14.3%) patients in both the blood and tissue DNA samples. In turn, the B19V genomic sequence Rabbit Polyclonal to TUSC3 was found in 5 out of 35 DNA samples derived from deceased subjects (Figure 1)in 1 case (2.9%) only in the thyroid tissue DNA sample and in 4 cases (11.4%) in the blood DNA samples. Open in a separate window Figure 1 Age and B19V infection rates of patients with non-autoimmune thyroid gland diseases (non-AITDs) and autoimmune thyroid gland diseases (AITDs), and deceased subjects as control; (A) dark symbols represent individuals with positive B19V infection (B19Vpos), and light grey symbols represent individuals without B19V infection (B19Vneg); the corresponding B19Vpos/neg ratio of each group is represented above the = 0.0076; KW) (Figure 2). The viral load in the one of the 35 nPCR B19V-positive thyroid tissue samples from the deceased subjects was 13.82 copies/g DNA. Cinnamyl alcohol In the whole blood of two individuals, it was less than 5 copies/g DNA (samples of additional two individuals were not tested due to the lack of material). Open in a separate window Figure 2 Comparison of numbers of B19V copies in the tissue and blood of patients with AITDs and non-AITDs. Light gray symbols show values under the quantification limit. Significance of differences was established using the Kruskal-Wallis (KW) test. 4. Discussion Despite the fact that B19V was discovered in.