In cases of abortion in cattle, histopathology and immunohistochemistry (IHC) using tissues from aborted fetuses are considered the definitive tests [6]. for abortion in cattle, resulting in drastic financial losses in the livestock industry attributable AAI101 to the abortion per se, loss of milk production, and costly control steps involving treatment and culling procedures [4,5]. Today, various diagnostic techniques are available for the detection of contamination. In cases of abortion in cattle, histopathology and immunohistochemistry (IHC) using tissues from aborted fetuses are considered the definitive assessments [6]. The polymerase chain reaction (PCR) is also used for the determination of parasite-specific nucleic acids in samples from aborted animals, such as brains and placenta [7]. However, the high costs, special gear requirements, and need for skilled persons when applying IHC and/or PCR restrict their use on a large scale [8]. Serological detection using different antibodies (Immunoglobulins G and M) is frequently used for diagnosis of infection in Vegfa different animals. Numerous serological tests have been used against including the indirect fluorescence antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA), and Western blotting. These assessments are regarded as efficient diagnostic assessments for antibody detection either in field or experimental animals when potent and specific antigens are used [9]. In addition, the detection of specific antibodies in sera of infected animals is frequently used to detect acute, sub-acute, or chronic contamination [10]. IgM and IgG-based detection are useful approaches to diagnosis and control because of their capability for differentiation between acute and chronic contamination, respectively [10,11]. Previous studies have established surface antigen 1 (SAG1), SAG1-related sequence (SRS2), and dense granule protein 6 (GRA6) or GRA7 to be the most frequently used antigens for diagnosis of infection, in either cattle or dogs [9,12]. In addition, anti-NcSAG1 antibodies have been reported in both acute and chronic contamination, whereas anti-NcGRA7 antibodies have been widely accepted as markers for acute contamination [9,13,14,15]. Moreover, the diagnostic and immunomodulatory properties of NcGRA6 have been reported [16,17]. On the other hand, the potential of lysate antigen (NLA) for detection of specific antibodies to contamination has also been reported. Therefore, many research groups are still using NLA as a standard antigen to validate newly developed antigens [9,12]. Herein, we proposed to establish a useful diagnostic tool for detection of specific antibodies against contamination in cattle based on the rapid AAI101 immunochromatographic test (ICT). Only one study has investigated the power of such an approach for diagnosis. Liao et al. (2005) [18] found that the NcSAG1-based ICT is useful for detection of infected sera from mice, dogs, and cattle. In addition, Pinheiro et al. (2005) presented dot-ELISA as a quick serologic method for detection of anti-antibodies in dogs [19]. However, since then, no other studies have been reported. Thus, the current study sought a convenient ICT by comparing various antigens, recombinant NcSAG1 (rNcSAG1), rNcGRA7, rNcGRA6, in addition to native lysate antigen. Our study provided novel AAI101 knowledge for the power of NcGRA7, NcGRA6, and NLA-based ICTs in the detection of sub-acute contamination in cattle. Also, the superiority of the NcSAG1-based ICT was proved through the capability of antibody detection in all positive control sera from different stages of infection and various animal species (mice: 2, 4, and 8 weeks post-infection (wpi); cattle: 4 and 8 wpi). This study is a great step toward the efficient diagnosis and control of in cattle because it offers various potent ICTs for rapid and on-site detection of infected cattle in the field. Nevertheless, a higher number of control samples from and PRU.